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Filtering and the Viability of Turkey Spermatozoa

Department of Poultry Science, Box 7608, North Carolina State University, Raleigh, NC 27695-7608; Phone: (919) 515-5554; FAX: (919) 515-7070

R. Whittaker

Prestage Farms, P. O. Box 438, Clinton, NC 28328

J. L. Grimes

The efficacy of turkey semen filtration was tested in six trials utilizing 63, 90, or 180 µm (M2, M3, CF) filters. Nonfiltered semen was compared to filtered semen using the modified ethidium bromide test for fresh and 24-hr stored semen in control buffer (100%) and stress buffer (20%) for all six trials.

In the first four trials, filtered semen sometimes had higher sperm viability (P<. 05) than nonfiltered semen. The response varied within and between trials with respect to filer, storage, and stress. In the other two trials, filtered semen shoed higher (P<. 05) sperm viability than nonfiltered semen when the semen was stored for 24 hr and stressed with the 20% buffer. Filtration may be appropriate for semen collected under problematic circumstances and semen that will be stored and/or may be stressed.

Key Words: Filtering • semen • spermatozoa • turkey • viability