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Improved In Vitro Methods for Determining Limestone and Oyster Shell Solubility

Department of Animal Science, University of Minnesota, St. Paul, MN 55108; Phone: (612) 625-1959; FAX: (612) 625-5789

Correspondence: Craig N. Coon

Experiments were conducted to determine the effect of changing the acid volume, acid normality, and incubation time on in vitro solubility values of various test calcium samples. A 200 mL volume of 0.2N HC1 solution combined with 2.0 g sample size and 10 min incubation time at 42°C produced a limestone solubility range of 20.96% to 78.15% for the samples used. This procedure produced a model for determining limestone or oyster shell weight loss (WL): WL = 1.809 + 0.601 x ln (pH change) (adjusted R²=. 961); solubility percentage was calculated as WL x 100/initial sample weight. This in vitro solubility procedure produced a higher limestone solubility range for the test samples, indicating its ability to better distinguish among different quality limestone and oyster shell sources. An in vitro assay using less than 2g of sample produced a higher standard error of the mean when large-particle limestone and oyster shell samples were tested.

Key Words: In vitro solubility method • limestone • oyster shell

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